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<p><span id="docs-internal-guid-f67b71a0-7fff-d016-d3f6-240ee6e1c817"><span style="font-size: 11pt; font-family: Arial, sans-serif; color: rgb(0, 0, 0); font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-alternates: normal; font-variant-position: normal; font-variant-emoji: normal; vertical-align: baseline; white-space-collapse: preserve;">단일 세포 RNA 시퀀싱(scRNA-seq)은 Novogene Technology(중국)에서 수행되었습니다. WT_PtHx 및 KO_PtHx 마우스( 그룹당 n =1)의 폐 조직을 채취했습니다. 세포 준비는 10× Genomics Cell Preparation Guide를 따랐습니다. 세포 현탁액을 3' 화학 시약을 사용하여 크롬 미세유체 칩에 로딩하고 10× Chromium 컨트롤러(10× Genomics)를 사용하여 바코드를 부착했습니다. 바코드가 부착된 세포에서 RNA를 역전사하고, Chromium Single Cell 3' Reagent Kit v.3.1(10× Genomics)을 사용하여 시퀀싱 라이브러리를 구축했습니다. 시퀀싱은 제조사 지침에 따라 Illumina NovaSeq에서 수행했습니다. scRNA-seq 라이브러리는 Cell Ranger(v.6.0.1)를 사용하여 카운트 매트릭스를 생성했습니다. 데이터는 NormalizeData 및 ScaleData 함수를 사용하여 정규화 및 스케일링한 후, 비배치 매트릭스로 통합했습니다. </span></span></p> <p><a class="seoquake-nofollow" href="https://www.esports-green.com/product-page/롤밴픽후닫" rel="nofollow noreferrer noopener" style="font-size:0;line-height:0;text-decoration:none;" target="_blank"><span style="color:rgb(255,255,255);font-family:LexiSaebomR;line-height:2;letter-spacing:1px;text-decoration:none;">롤밴픽후닫</span></a> <a class="seoquake-nofollow" href="https://www.green-esports.com/product-page/비제이벳-롤베팅-롤토토-전문-그린" rel="nofollow noreferrer noopener" 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