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<p>ChIP-PCR 분석 결과, ZFP64는 2번 부위(-177~-169), 3번 부위(-207~-199), 4번 부위(-287~-264)에 결합하는 것이 확인되었지만, 1번 부위(-74~-66) 또는 5번 부위(-391~-383)에는 결합하지 않는 것으로 나타났습니다. 이는 ZFP64가 ZIP4를 직접적으로 전사 조절한다는 것을 더욱 뒷받침합니다( 그림 3I ). 대식세포 조절에서 ZFP64의 역할을 더 자세히 조사하기 위해, 우리는 악액질이 적은(shZIP4) 또는 악액질이 심한(shV) 종양 세포에서 ZFP64를 과발현시켰습니다. 이들 세포에서 유래한 배양액은 대식세포의 화학주성을 유의하게 증가시키고 CD206 발현을 증가시켜, 종양 관련 대식세포의 모집 및 분극화를 촉진함을 나타냈습니다( 그림 3J 및 3K). 특히, CSF1R 차단 항체로 처리했을 때 이러한 표현형이 대부분 사라졌는데( 그림 3L 및 3M), 이는 ZFP64가 CSF1 신호전달을 통해 효과를 발휘함을 시사합니다. 종합적으로, 이러한 결과는 ZFP64가 CSF1을 통해 대식세포 모집 및 분극화를 촉진하여 종양 미세환경(TME)에서 대식세포 유래 GDF15의 증가에 기여함을 보여줍니다. <a class="seoquake-nofollow" href="https://mukssa-news.com/" rel="nofollow noreferrer noopener" style="font-size:0;line-height:0;text-decoration:none;" target="_blank"> <span style="color:rgb(255,255,255);font-family:LexiSaebomR;line-height:2;letter-spacing:1px;text-decoration:none;">먹튀검증</span></a> <a class="seoquake-nofollow" href="https://xn--910br1nf9g2g92y.com/" rel="nofollow noreferrer 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